农业物联网在新疆棉花产业中的应用与发展——以农八师一五〇团为例

为解决新疆农业物联网综合利用程度不高的问题,文中介绍了目前农业物联网中关键技术研究进展,同时以新疆农八师一五O团为例,对其棉花产业对农业物联网的应用进行了分析,并针对农业物联网在新疆的应用过程出现的问题进行了分析,提出了相应的解决策略。最后对一五O团的棉花产业的发展进行了总结。     

小型生物质直燃炉结构设计

基于目前现有生物质燃烧炉的燃烧效率低,且燃烧过程中焦油生成过多等问题。以高效率燃烧方式的薄层燃烧为该小型生物质直燃炉的主要燃烧方式,大大提高燃烧效率的同时,有效较低焦油与烟尘的产生,并且采用螺杆输送装置对炉膛进行持续供料,实现稳定的不间断燃烧,为成功解决农村生物质燃料浪费及环境污染起到积极作用,为新形势下的能源可持续发展提供思路。     

沼液追肥对制种玉米产量与土壤化学性质的影响

研究沼液作追肥对制种玉米产量及土壤化学性质的影响。文章以制种玉米为试验材料,采用计随机区组试验设,研究沼液追肥对制种玉米经济性状、产量、土壤化学性质、经济效益的影响。结果表明,T1(沼液1500kg·hm-2+142.50 kg N·hm-2)有机质含量最高,与其他处理间达到了显著差异;T1的土壤速效钾含量最高,达12.67 g·kg-1,与CK(150 kg N·hm-2),T2(沼液22500 kg·hm-2+138.75 kg N·hm-2)间达到了显著差异;土壤速效磷含量,土壤p H值,土壤电导率差异不显著;不同处理对穗行数,行粒数、穗粒重的影响差异不显著,T3(沼液30000 kg·hm-2+135 kg N·hm-2)千粒重最高,与CK,T2,T4(沼液37500 kg·hm-2+131.25 kg N·hm-2)间差异达到了显著水平;T2的产量,产值,经济效益最高。沼液做追肥可提高产量,降低生产成本,但必须和化肥配合施用。     

农田平地机激光发射平台调平控制系统设计

为使农田平地机双源激光定位系统的激光发射平台检测数据更精确、调平实时性更好,设计了激光发射平台自动调平控制系统。该系统采用三脚支撑的平台支撑方式,利用倾角传感器检测平台的倾斜度,通过步进电机驱动平台3条支腿伸缩从而实现平台调平。通过加入倾角传感器数据处理算法和基于RBF神经网络的智能PID控制算法,激光发射平台检测的数据更精确,能满足双激光源定位系统的要求,使农田平地机平地作业更精确。     

Prokaryotic Expression and Antigenicity Analysis of Porcine Japenese Encephalitis Virus Envelope Protein Domain Ⅲ

In order to analyze the antigenicity of porcine Japanese encephalitis virus (JEV) E protein domain Ⅲ, which was expressed by pET-28a vector with His-tag and purified through Ni-NTA, the BALB/c mice were immunized with the purified protein.We identified the antigenicity of domain Ⅲ of E protein and the anti-mice and anti-porcine JEV E Ⅲ protein specific antibody titers by SDS-PAGE, Western blotting, indirect ELISA and IFA.SDS-PAGE results showed the expressed target protein existed mainly in the form of inclusion body.Western blotting, ELISA test results showed that the protein had good reactivity with anti-serum.The mice immunized with the purified JEV E Ⅲ protein generated 1×10⁵ anti-JEV E Ⅲ protein specific antibody titers by ELISA, and the porcine immunized with the porcine JEV generated 5.1×10⁴ anti-JEV specific antibody titers.The IFA results showed that JEV E Ⅲ protein anti-serum could identify JEV antigen.The above results showed that the recombinant JEV E Ⅲ had good antigenicity.These results provided important basis for development of diagnostic antigen for JEV.     

Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

氮肥配施生物炭和脲酶抑制剂对夏玉米-冬小麦产量及氮素利用的影响

于2019—2021年采用再裂区设计,设置氮肥、生物炭和脲酶抑制剂3个因素,主处理设5个氮水平：0、75、150、225 kg·hm^-2和300 kg·hm^-2,副处理设2个生物炭水平：0 t·hm^-2和7.5 t·hm^-2,副副处理设2个脲酶抑制剂水平：0%和2%,共20个处理,研究氮肥配施生物炭和脲酶抑制剂对夏玉米-冬小麦轮作体系作物产量和氮肥吸收利用的影响。结果表明,施用生物炭显著提高夏玉米和冬小麦产量、植株氮素吸收量、氮肥表观利用率、氮素收获指数以及夏玉米地上部生物量,较不施生物炭处理分别增加4.4%和2.9%、2.3%和3.0%、25.8%和13.5%、4.9%和6.1%、4.5%;氮肥单独配施生物炭可显著提高夏玉米和冬小麦产量、植株氮素吸收量和氮肥表观利用率,且氮肥和生物炭具有显著的交互效应。施用脲酶抑制剂显著增加夏玉米植株氮素吸收量和氮肥表观利用率,较不施脲酶抑制剂处理分别提高1.5%和3.0%;氮肥单独配施脲酶抑制剂可提高夏玉米植株氮素吸收量和氮肥表观利用率,但氮肥与脲酶抑制剂无显著...     

p38MAPK信号通路选择性调节FSH对甾体生成 的诱导作用研究

利用乙烯雌酚（DES）处理23日龄SD大鼠，分离卵巢颗粒细胞(GC)进行无血清培养。结果表明，FSH (50ng/m1) 处理GC，可迅速激活有丝分裂原蛋白激酶（p38 MAPK），FSH处理5min便可观察到磷酸化的p38 MAPK；FSH处理30min，磷酸化的p38 MAPK水平达到最高;向培养液中加入H89(蛋白激酶A抑制剂，10μM)，则显著抑制了FSH对p38 MAPK的激活作用，提示这种激活作用依赖于蛋白激酶A(PKA)。用 SB203580(p38MAPK抑制剂，20μM)抑制p38 MAPK激活，则进一步提高了FSH对孕酮和甾体生成快速调节蛋白（StAR）的诱导作用，同时降低了FSH对雌激素生成的促进作用(p<0.01)。RT-PCR结果显示：抑制p38 MAPK活性后，FSH对StAR mRNA刺激作用明显增强，但对细胞色素P450芳香化酶（P450arom）mRNA的诱导作用却减弱了(p<0.05)。激光共聚焦和蛋白印迹结果显示：在GC中，StAR蛋白主要分布在线粒体中；与对照组相比，FSH显著提高了StAR的荧光强度和蛋白水平；抑制p38MAPK活性则增强了FSH对StAR蛋白表达的诱导作用。     

A Fast One-Step Scanning Thinning Algorithm

A new quick thinning algorithm was presented in this paper,which assigneddifferent weights to different pixels near the skeleton pixels and rules out the im possible pixels in thethinning procedure. It needs neither iteration on pixel panel nor detection on every pixel such thatthe time consumed is greatly lessened. A filling algorithm is also offered for better results.Experiment on variety of binary patterns showed that it could get both a high speed and a goodskeleton shape compared with other algorithms,This new algorithm reaches perfect result.